Lijun Cao - PhD Final Oral Exam

Monday, April 29, 2019 - 9:30am to 12:00pm

Major Professors:  Wei Wang and Steve Whitham 

Home Department:  Plant Pathology and Microbiology

Title of Defense:
   Bidirectional Interaction between Soybean Root Circadian Clock and Soybean Cyst Nematode (SCN)

   Plant circadian clock has emerged as a cardinal signaling hub integrating diverse pathogenic and immune signals to gate plant immunity and coordinate metabolic needs for growth. However, almost all the discoveries so far are made through studies on leaf pathogens. Virtually nothing is known about the interactions between the root circadian clock and root pathogens. To fill this critical knowledge gap, this research initiates the first comprehensive study on the bidirectional interactions between soybean root circadian clock and an economically important root pathogen, soybean cyst nematodes (SCN). By using RNA-mediated oligonucleotide annealing, selection, and ligation with next-generation sequencing (RASL-seq) and a live imaging system, I found two root circadian components targeted by SCN. On the other hand, using mutant-clock soybean hairy root lines and RNA-seq, I found the key soybean resistant loci were clock regulated.